![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K1023006:Design
Designed by: Michiel Karrenbelt Group: iGEM13_Wageningen_UR (2013-09-17)
actinGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1308
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1613
Illegal XhoI site found at 2068 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1349
Illegal AgeI site found at 1807 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1735
Illegal BsaI.rc site found at 2188
Design Notes
The construct contains a GFP fused to the actin gene. The GFP is fused to the N-terminal site oof the actin gene.
Source
The actin encoding gene was isolated from A. niger by PCR as it is already present in the organism.