Composite

Part:BBa_K1023006:Design

Designed by: Michiel Karrenbelt   Group: iGEM13_Wageningen_UR   (2013-09-17)

actinGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1308
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1613
    Illegal XhoI site found at 2068
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1349
    Illegal AgeI site found at 1807
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1735
    Illegal BsaI.rc site found at 2188


Design Notes

The construct contains a GFP fused to the actin gene. The GFP is fused to the N-terminal site oof the actin gene.

Source

The actin encoding gene was isolated from A. niger by PCR as it is already present in the organism.

References